| 质粒名称: | pBabe puro Myr HA PIK3CA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12523 |
| 存储实验室: | Jean Zhao |
|
载体骨架基本信息 |
|
| 载体名称: | pBabe puro |
| 载体出品公司: | Available at Addgene (plasmid #1764) |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5150 bp |
| 完整质粒大小: | 8450 bp |
| 载体类型: | 哺乳细胞表达，Retroviral |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PIK3CA Myr |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3300 bp |
| 基因又名: | phosphoinositide 3-kinase;PI3K;p110 alpha |
| 基因突变: | Myristoylation. Constitutively active allele; I143V |
| 标签/融合蛋白: |
Myr (N terminal on insert)
HA (N terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 3' 克隆位点: | SalI (未破坏) |
| 5' 测序引物 | pBABE 5' |
| 3' 测序引物 | pBABE-3 |
|
文献引用方法 |
|
| 材料和方法部分: | pBabe puro Myr HA PIK3CA was a gift from Jean Zhao (爱迪基因 plasmid # 12523) |
| 参考文献部分: | The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Zhao JJ, Liu Z, Wang L, Shin E, Loda MF, Roberts TM. Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8. 10.1073/pnas.0508988102 PubMed 16339315 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBabe puro Myr HA PIK3CA 8451 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBabe puro Myr HA PIK3CA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8451)
AUTHORS Zhao JJ, Liu Z, Wang L, Shin E, Loda MF, Roberts TM
TITLE The oncogenic properties of mutant p110alpha and p110beta
phosphatidylinositol 3-kinases in human mammary epithelial cells.
JOURNAL Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8.
PUBMED 16339315
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pBabe puro Myr HA PIK3CA|
FEATURES Location/Qualifiers
source 1..8451
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 934..1350
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1318..1334
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 1424..1450
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(4690..4710)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4695..5024
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4875..5010
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4937..4956
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5034..5633
/codon_start=1
/gene="pac from Streptomyces"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
primer_bind complement(5034..5053)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5530..5550
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5732..6325
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(6526..6543)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6697..7285)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6777..6796)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7463..8323)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8086..8105
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8324..8428)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt
901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc
1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt
1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt
1321 tatccagccc tcactccttc tctaggcgcc ggccggatct accatggggt cttcaaaatc
1381 taaaccaaag gaccccagcc agcgccggcg caggatccga ggttacccat acgacgtgcc
1441 agattacgcc agatcccagt gtggtggtac gtaccctcca agaccatcat caggtgaact
1501 gtggggcatc cacttgatgc ccccaagaat cctagtagaa tgtttactac caaatggaat
1561 gatagtgact ttagaatgcc tccgtgaggc tacattaata accataaagc atgaactatt
1621 taaagaagca agaaaatacc ccctccatca acttcttcaa gatgaatctt cttacatttt
1681 cgtaagtgtt actcaagaag cagaaaggga agaatttttt gatgaaacaa gacgactttg
1741 tgaccttcgg ctttttcaac cctttttaaa agtaattgaa ccagtaggca accgtgaaga
1801 aaagatcctc aatcgagaaa ttggttttgc tatcggcatg ccagtgtgtg aatttgatat
1861 ggttaaagat ccagaagtac aggacttccg aagaaatgtt ctgaacgttt gtaaagaagc
1921 tgtggatctt agggacctca attcacctca tagtagagca atgtatgtct atcctccaaa
1981 tgtagaatct tcaccagaat tgccaaagca catatataat aaattagata aagggcaaat
2041 aatagtggtg atctgggtaa tagtttctcc aaataatgac aagcagaagt atactctgaa
2101 aatcaaccat gactgtgtac cagaacaagt aattgctgaa gcaatcagga aaaaaactcg
2161 aagtatgttg ctatcctctg aacaactaaa actctgtgtt ttagaatatc agggcaagta
2221 tattttaaaa gtgtgtggat gtgatgaata cttcctagaa aaatatcctc tgagtcagta
2281 taagtatata agaagctgta taatgcttgg gaggatgccc aatttgatgt tgatggctaa
2341 agaaagcctt tattctcaac tgccaatgga ctgttttaca atgccatctt attccagacg
2401 catttccaca gctacaccat atatgaatgg agaaacatct acaaaatccc tttgggttat
2461 aaatagtgca ctcagaataa aaattctttg tgcaacctac gtgaatgtaa atattcgaga
2521 cattgataag atctatgttc gaacaggtat ctaccatgga ggagaaccct tatgtgacaa
2581 tgtgaacact caaagagtac cttgttccaa tcccaggtgg aatgaatggc tgaattatga
2641 tatatacatt cctgatcttc ctcgtgctgc tcgactttgc ctttccattt gctctgttaa
2701 aggccgaaag ggtgctaaag aggaacactg tccattggca tggggaaata taaacttgtt
2761 tgattacaca gacactctag tatctggaaa aatggctttg aatctttggc cagtacctca
2821 tggattagaa gatttgctga accctattgg tgttactgga tcaaatccaa ataaagaaac
2881 tccatgctta gagttggagt ttgactggtt cagcagtgtg gtaaagttcc cagatatgtc
2941 agtgattgaa gagcatgcca attggtctgt atcccgagaa gcaggattta gctattccca
3001 cgcaggactg agtaacagac tagctagaga caatgaatta agggaaaatg acaaagaaca
3061 gctcaaagca atttctacac gagatcctct ctctgaaatc actgagcagg agaaagattt
3121 tctatggagt cacagacact attgtgtaac tatccccgaa attctaccca aattgcttct
3181 gtctgttaaa tggaattcta gagatgaagt agcccagatg tattgcttgg taaaagattg
3241 gcctccaatc aaacctgaac aggctatgga acttctggac tgtaattacc cagatcctat
3301 ggttcgaggt tttgctgttc ggtgcttgga aaaatattta acagatgaca aactttctca
3361 gtatttaatt cagctagtac aggtcctaaa atatgaacaa tatttggata acttgcttgt
3421 gagattttta ctgaagaaag cattgactaa tcaaaggatt gggcactttt tcttttggca
3481 tttaaaatct gagatgcaca ataaaacagt tagccagagg tttggcctgc ttttggagtc
3541 ctattgtcgt gcatgtggga tgtatttgaa gcacctgaat aggcaagtcg aggcaatgga
3601 aaagctcatt aacttaactg acattctcaa acaggagaag aaggatgaaa cacaaaaggt
3661 acagatgaag tttttagttg agcaaatgag gcgaccagat ttcatggatg ctctacaggg
3721 ctttctgtct cctctaaacc ctgctcatca actaggaaac ctcaggcttg aagagtgtcg
3781 aattatgtcc tctgcaaaaa ggccactgtg gttgaattgg gagaacccag acatcatgtc
3841 agagttactg tttcagaaca atgagatcat ctttaaaaat ggggatgatt tacggcaaga
3901 tatgctaaca cttcaaatta ttcgtattat ggaaaatatc tggcaaaatc aaggtcttga
3961 tcttcgaatg ttaccttatg gttgtctgtc aatcggtgac tgtgtgggac ttattgaggt
4021 ggtgcgaaat tctcacacta ttatgcaaat tcagtgcaaa ggcggcttga aaggtgcact
4081 gcagttcaac agccacacac tacatcagtg gctcaaagac aagaacaaag gagaaatata
4141 tgatgcagcc attgacctgt ttacacgttc atgtgctgga tactgtgtag ctaccttcat
4201 tttgggaatt ggagatcgtc acaatagtaa catcatggtg aaagacgatg gacaactgtt
4261 tcatatagat tttggacact ttttggatca caagaagaaa aaatttggtt ataaacgaga
4321 acgtgtgcca tttgttttga cacaggattt cttaatagtg attagtaaag gagcccaaga
4381 atgcacaaag acaagagaat ttgagaggtt tcaggagatg tgttacaagg cttatctagc
4441 tattcgacag catgccaatc tcttcataaa tcttttctca atgatgcttg gctctggaat
4501 gccagaacta caatcttttg atgacattgc atacattcga aagaccctag ccttagataa
4561 aactgagcaa gaggctttgg agtatttcat gaaacaaatg aatgatgcac atcatggtgg
4621 ctggacaaca aaaatggatt ggatcttcca cacaattaaa cagcatgcat tgaactgagt
4681 cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
4741 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
4801 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
4861 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
4921 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
4981 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt accatgaccg
5041 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
5101 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
5161 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
5221 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
5281 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
5341 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
5401 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
5461 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
5521 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
5581 agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
5641 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg ataaaataaa
5701 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
5761 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
5821 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
5881 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
5941 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
6001 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
6061 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
6121 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
6181 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
6241 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
6301 tacccgtcag cgggggtctt tcatttccga cttgtggtct cgctgccttg ggagggtctc
6361 ctctgagtga ttgactaccc gtcagcgggg gtcttcacat gcagcatgta tcaaaattaa
6421 tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc
6481 aacgcgcggg gagaggcggt ttgcgtattg gcgctcttcc gcttcctcgc tcactgactc
6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
7381 cacctagatc cttttaaatt aaaaatgaag tttgcggccg caaatcaatc taaagtatat
7441 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
7501 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
7561 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
7621 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
7681 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
7741 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
7801 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
7861 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
7921 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
7981 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
8041 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
8101 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
8161 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
8221 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
8281 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
8341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
8401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca c
//
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